kristian helin  (Addgene inc)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Classification of Paediatric Celiac Disease Using RNA Sequencing and Real‐Time PCR of Duodenal Biomarkers

doi: 10.1111/jcmm.70854

Figure Lengend Snippet: (a–d) Expression of (a) CYP3A4 , (b) LCT , (c) CXCL11 , and (d) E2F5 , normalised to the lowest expression in the whole dataset and presented on a log 2 scale. The grey marker in group BiopsyDiag represents the misclassified biopsy from case 51, and the grey marker in group TGdiag represents the misclassified biopsy from case 55. From left to right in each plot, the groups are: Not CD, Normalised CD, Potential CD without progression, Potential CD with progression, Potential CD with high anti‐TG2 and progression, Biopsy‐based CD diagnosis, Anti‐TG2 based CD diagnosis, and Marsh 3 histopathology and negative anti‐TG2.

Article Snippet: E2F5 , E2F transcription factor 5 , 8q21.2 , Hs00231092_m1.

Techniques: Expressing, Marker, Biomarker Discovery, Histopathology

Journal: Translational Andrology and Urology

Article Title: Fu Zheng Xiao Yu San Jie Decoction affects the proliferation of renal cell carcinoma via regulating E2F5 gene

doi: 10.21037/tau-2025-aw-739

Figure Lengend Snippet: Potential target genes of FZXYJD in E2F family were predicted. (A) E2F5 has low expression in ccRCC. (B) Patients had poorer survival with low expression of E2F5 in ccRCC. (C-F) FZXYJD can increase the expression level of E2F5 in ccRCC cell line. *, P<0.05; **, P<0.01; ***, P<0.001. Control, cultured in SD rat serum. LD, cultured in FZXYJD lower dose group serum. MD, cultured in FZXYJD medium dose group serum. HD, cultured in FZXYJD high-dose group serum. ccRCC, clear cell renal cell carcinoma; FZXYSJD, Fu Zheng Xiao Yu San Jie Decoction; HD, high-dose; HR, hazard ratio; KIRC, kidney renal clear cell carcinoma; LD, low-dose; MD, medium-dose; N, normal; SD, Sprague-Dawley; T, tumor; TPM, transcripts per million.

Article Snippet: It was then incubated overnight at 4 °C with the primary antibodies: E2F5 (1:1,000, Catalog: D197169, BBI, Shanghai, China) and β-Actin (1:1,000, Catalog: ab8227, Abcam, Shanghai, China).

Techniques: Expressing, Control, Cell Culture

Journal: Translational Andrology and Urology

Article Title: Fu Zheng Xiao Yu San Jie Decoction affects the proliferation of renal cell carcinoma via regulating E2F5 gene

doi: 10.21037/tau-2025-aw-739

Figure Lengend Snippet: E2F5 was involved in FZXYJD suppression of ccRCC cells proliferation. (A) Western blot and RT-qPCR were performed to analyze E2F5 expression in si E2F5 -transfected ccRCC cell line. β-actin was used as an internal quantitative control. (B) CCK-8 assay. (C) Cell colony formation assay [IPHONE X PRO MAX] (magnification: 1×; crystal violet stained). *, P<0.05; **, P<0.01; ***, P<0.001. CCK-8, Cell Counting Kit-8; ccRCC, clear cell renal cell carcinoma; FZXYSJD, Fu Zheng Xiao Yu San Jie Decoction; NC, negative control; OD, optical density; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; si E2F5 , small interfering RNA targeting E2F5 .

Article Snippet: It was then incubated overnight at 4 °C with the primary antibodies: E2F5 (1:1,000, Catalog: D197169, BBI, Shanghai, China) and β-Actin (1:1,000, Catalog: ab8227, Abcam, Shanghai, China).

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Transfection, Control, CCK-8 Assay, Colony Assay, Staining, Cell Counting, Negative Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Small Interfering RNA

Journal: Translational Andrology and Urology

Article Title: Fu Zheng Xiao Yu San Jie Decoction affects the proliferation of renal cell carcinoma via regulating E2F5 gene

doi: 10.21037/tau-2025-aw-739

Figure Lengend Snippet: Three-dimensional and two-dimensional docking patterns and interactions of FZXYSJD identified components with the E2F5 protein. FZXYSJD, Fu Zheng Xiao Yu San Jie Decoction.

Article Snippet: It was then incubated overnight at 4 °C with the primary antibodies: E2F5 (1:1,000, Catalog: D197169, BBI, Shanghai, China) and β-Actin (1:1,000, Catalog: ab8227, Abcam, Shanghai, China).

Techniques: